BAC-YAC LIBRARIES AVAILABLE AT THE INRA BAC-YAC RESOURCE CENTER

BAC-YAC LIBRARIES AVAILABLE AT THE INRA BAC-YAC RESOURCE CENTER

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Porcine BACs	Laboratoire de Radiobiologie et d'Etude du Génome (LREG)	Skin fibroblasts from a Large White male pig homozygous for the SLA H01 haplotype	HindIII	pBeloBAC11	E. Coli DH10B	Rogel-Gaillard C. et al. Cytogenet Cell Genet 1999, vol.85, n°3-4 pp205-211	107520	135	5X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Porcine YACs	Laboratoire de Radiobiologie et d'Etude du Génome (LREG)	Skin fibroblasts from a Large White male pig homozygous for the SLA H01 haplotype	?	pYAC4	?	Rogel-Gaillard C. et al. Mamm Genome 1997 Mar;8(3):186-92	33792	280	3X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Bovine BACs	Laboratoire de Génétique Biochimique et Cytogénétique (LGBC)	Fibroblasts from a Holstein male fœtus (son of de Pen Col Duster)	HindIII	pBeloBAC11	E. Coli DH10B	In progress	105984	115-120	4X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Bovine YACs	Texas A&M University	Skin fibroblasts from a Hereford male	EcoRI	pYAC4	AB1380	Libert F., Genomics 18, 270-276 (1993)	21500	750	6X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Ovine BACs	Laboratoire de Génétique Biochimique et Cytogénétique (LGBC)	Brain cells from a Romanov ram affected with natural scrapie	HindIII	pBeloBAC11	E. Coli DH10B	Vaiman D et al. Mamm Genome 1999 Jun;10(6):585-7	89280	123	3.4X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Rabbit BACs	Laboratoire de Radiobiologie et d'Etude du Génome (LREG)	White blood cells from a New-Zealand sire rabbit (homozygous for the class II region of the RLA complex)	HindIII	pBeloBAC11	E. Coli DH10B	In progress	84480	100-110	3.3X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Caprine BACs	Laboratoire de Génétique Biochimique et Cytogénétique (LGBC)	Fibroblasts from a male carrying a 6;15 Robertsonian translocation	HindIII	pBeloBAC11	E. Coli DH10B	Schibler L, et al. Mamm Gen. 1998 Feb;9(2):119-24	61440	153	3.3X

Library	Origin	Source of material	Cloning site	Vector	Bacterial strain	Reference	Number of clones	Average insert size (kb)	Genomic equivalents
Equine BACs	Laboratoire de Génétique Biochimique et Cytogénétique (LGBC)	Male fibroblasts	HindIII	pBeloBAC11	E. Coli DH10B	Godard S et al. Mamm Gen 1998 Aug;9(8):633-7	108288	In progress	In progress